Online Mode of Teaching and Learning Process in Engineering Discipline: Teacher Perspective on Challenges Faced and Recommendations

The COVID-19 pandemic has affected people from almost every walk of life in general and academia in particular. It had a huge impact on teaching and learning resulting in a sudden shift from classroom and face-to-face teaching to distance and online teaching and learning. This sudden shift created a lot of ruckuses in the teaching of engineering disciplines. This study is pertinent to the examination of faculty perceptions of online teaching in Pakistani universities and the obstacles they face in teaching engineering students through the online mode during this pandemic. The research takes a quantitative and sample survey approach. A Google form questionnaire was used to collect the data from a sample of 91 faculty members from the engineering discipline of different universities in Pakistan during 2022. According to the study's findings, faculty generally have a favorable opinion of virtual teaching in the context of COVID-19 for closing the achievement gap and guiding students' futures in difficult times. However, they ran into a number of challenges when teaching online, including technological difficulties, problems with student participation, challenges with online tests and assessments, etc. The results of this study will urge educational institutions and policymakers to use the most up-to-date instructional methodologies and offer teachers ongoing professional development in order to improve the quality of online teaching, learning, and assessment in universities. Previous studies discussed a number of obstacles faced by students in virtual teaching in higher education, overlooking the perception and challenges faced by the engineering faculty. The present study replenishes this gap.

Point Prevalence Survey of Antimicrobial Use during the COVID-19 Pandemic among Different Hospitals in Pakistan: Findings and Implications.

The COVID-19 pandemic has significantly influenced antimicrobial use in hospitals, raising concerns regarding increased antimicrobial resistance (AMR) through their overuse. The objective of this study was to assess patterns of antimicrobial prescribing during the current COVID-19 pandemic among hospitals in Pakistan, including the prevalence of COVID-19. A point prevalence survey (PPS) was performed among 11 different hospitals from November 2020 to January 2021. The study included all hospitalized patients receiving an antibiotic on the day of the PPS. The Global-PPS web-based application was used for data entry and analysis. Out of 1024 hospitalized patients, 662 (64.64%) received antimicrobials. The top three most common indications for antimicrobial use were pneumonia (13.3%), central nervous system infections (10.4%) and gastrointestinal indications (10.4%). Ceftriaxone (26.6%), metronidazole (9.7%) and vancomycin (7.9%) were the top three most commonly prescribed antimicrobials among surveyed patients, with the majority of antibiotics administered empirically (97.9%). Most antimicrobials for surgical prophylaxis were given for more than one day, which is a concern. Overall, a high percentage of antimicrobial use, including broad-spectrums, was seen among the different hospitals in Pakistan during the current COVID-19 pandemic. Multifaceted interventions are needed to enhance rational antimicrobial prescribing including limiting their prescribing post-operatively for surgical prophylaxis.

Changing Food Patterns during the Pandemic: Exploring the Role of Household Dynamics and Income Stabilization Strategies

COVID-19 still looms as the largest risk to the agriculture, energy, and health sectors, threatening sustainable global economic development. The literature shows that the COVID-19 pandemic can divert governments' attention away from climate change, renewable energy, and food security challenges that are necessary to address for sustainable economic growth. The COVID-19 pandemic has consistently influenced environmental behaviors, as it has primarily decreased income levels and disrupted food systems worldwide. This study examined the impacts of COVID-19 on food consumption patterns, food diversity, and income challenges and explored the factors affecting food consumption patterns during the pandemic. The data collected through an online survey from 1537 Chinese households were analyzed through a paired t-test, a mixed-design ANOVA, and a logistic regression analysis. The results revealed that the consumption of the majority of individual food commodities decreased during the COVID-19 pandemic. Among the individual food items, the consumption of pork witnessed the greatest decrease during the COVID-19 pandemic compared to the normal period. The decrease in food diversity was higher for the households whose income was affected compared to the households whose income was not affected during the COVID-19 pandemic. Furthermore, the consumption quantities of various food groups declined more for highly income-affected households than for medium and slightly affected households during the pandemic. Households that adopted a dissaving income-stabilizing strategy were 47% points more likely to maintain their food consumption patterns during the pandemic. Farmers were 17% points and 19% points less likely to suffer worsened food consumption compared to self-employed and wage workers, respectively, during the pandemic. Thus, self-production methods such as kitchen gardening can assist households to maintain and improve their consumption of food commodities during the COVID-19 pandemic.

Do capital markets reward corporate climate change actions? Evidence from the cost of debt

As a result of recurring natural disasters caused by climate change, firms are under enormous pressure to reconsider their environmental footprints. However, whether or not investors reward firms' climate change actions remains a topic of considerable debate. Using a sample of S&P 500 companies over the period 2005-2020, we hypothesise and find a significant negative relationship between climate change actions and the cost of debt, indicating that investors indeed reward corporate climate efforts in the form of lower cost funds. This relationship exists in both environmentally sensitive and non-sensitive industries and remains negative and statistically significant even after controlling for the impact of the ongoing pandemic (COVID-19). The findings are robust to the use of alternative measures for our variables, alternative estimation methods and after controlling for endogeneity issues. We interpret our findings within the decision-usefulness and stakeholder-agency theories that suggest that non-financial information on firms' environmental performance is becoming increasingly important when borrowers' creditworthiness is assessed. Our study offers important regulatory and academic policy implications.

Maintaining Quality of Life during the Pandemic: Managing Economic, Social, and Health Well-Being Amid the COVID-19 Crisis of Agricultural Entrepreneurs

Every sphere of life is being impacted by COVID-19, but little is known about how the pandemic is affecting agricultural entrepreneurs' quality of life in developing nations. Therefore, this study examined how COVID-19 affected agricultural entrepreneurs' quality of life by utilizing the data collected from 220 females and 1501 males through multistage purposive and random sampling methods. The dataset was analyzed using ordered logistic regression and principal component analysis. The study results indicated that female agricultural entrepreneurs' quality of life was more adversely affected than that of male agricultural entrepreneurs during COVID-19. The findings also showed that male and female agricultural entrepreneurs with a lower socioeconomic status were more severely impacted than male and female agricultural entrepreneurs with a higher socioeconomic status. More female agricultural entrepreneurs compared to males reported that the pandemic had a negative impact on their mental health. More than two-thirds of both male and female agricultural entrepreneurs reported that the pandemic had a negative impact on their ability to purchase both food and non-food items. Similar to this, a sizable majority of people of both sexes stated that COVID-19 had an impact on their ability to access medical facilities. The results also showed that COVID-19 had a greater impact on married agricultural entrepreneurs' quality of life than on single people. Therefore, a paradigm shift in agricultural policy is required for the pandemic response to account for COVID-19's various effects on different genders and socioeconomic groups in rural areas.

Prediction of Covid-19 infection severity using ABO blood group types and Rh factor

Background and Objective: Biological markers for the prediction of acquiring Covid-19 risk are deficient and there is a dire need of immediate research data. The objective of the study was to predict the link of ABO blood group types along with Rh factor distribution with the severity of Covid-19. Methods: This was an observational cross-sectional survey conducted in medicine department of Pakistan Ordnance Factory Hospital, Wah Cantt Pakistan, from August 2020 to December 2020 after approval of IRB. Participants tested positive for presence of Covid-19 infection by polymerase chain reaction (PCR) were included in the study. Covid-19 infection severity was measured through mild, moderate and severe disease categories and analyzed. ABO blood group and Rh subgroups data for all the Covid-19 infected patients were obtained from the laboratory section of the hospital and analyzed. Data was entered in SPSS v 26 and analyzed. Cox regression model was used to find out the severity of Covid-19. Results: Total 248 patients were included;75% patients were male and 25% were females. The mean age of the patients was 52.77±15.58 years. A very significant association was found between ABO blood group types, Rh factor antigen and severity of Covid-19 (p=0.001). When stratified ABO, Rh antigen blood group with health status of all patients there was a very significant association between them (p=0.013). An insignificant association between male and female odds ratio of ABO blood group types but blood group B, Rh positive antigen was more susceptible in Covid-19 positive patients. Conclusion: There is a link between ABO blood group types along with Rh factor antigen (B+ and O+) with the severity of Covid-19 positive patients. ABO blood group types and Rh factor can be used as a potential marker/tool to predict the susceptibility of acquiring Covid-19 infection as well as for severity of the infection.

The Impact of COVID-19 on the Psychological Well-Being of Surgeons in Pakistan: A Multicenter Cross-Sectional Study

Introduction The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic left a profound and pervasive impact on the healthcare infrastructure on a global scale. Since its onset, the pattern of reported cases and its associated mortality had shown variability with intermittent peaks causing a significant effect on the psychological well-being of the surgeons of Pakistan. The aim of this study was to assess the effects of the COVID-19 pandemic on the mental well-being of surgeons in Pakistan. Methods This multicenter cross-sectional study was carried out to assess the impact of COVID-19 on the psychological well-being of surgeons in Pakistan. The validated Self-Reporting Questionnaire-20 (SRQ-20) tool was circulated electronically via Google Forms (Google, Inc., Mountain View, CA, USA) in the practicing surgical fraternity across all five regions of Pakistan, i.e., Sindh, Punjab, Baluchistan, Khyber Pakhtunkhwa (KPK), and Azad Jammu and Kashmir (AJK). Results This study showed that the female gender, having fewer years of working experience, non-satisfaction with the available personal protective equipment (PPE), and working in the public sector were the factors affecting the psychological well-being of surgeons during the pandemic. Conclusion Considering the continuous rise in new cases during the ongoing pandemic, the mental health of surgeons working in low- and middle-income countries (LMIC) such as Pakistan has been significantly affected. There is an undeniable need to pay close attention to their psychological well-being. Measures need to be undertaken to ensure their physical and mental health and wellness.

The Role of Service Recovery in Post-purchase Consumer Behavior During COVID-19: A Malaysian Perspective

The purpose of this study is to investigate the incidence of service failure in rendering service process during COVID-19. It further explores the outcomes of service recovery offered to customers in case of service failure. Like other businesses, webstores have also faced the challenges in their efforts to satisfy their customers during COVID-19. Service failure has increased due to unexpected circumstances produced by this pandemic. It has become necessary for the webstores to retain their dissatisfied customers by reconsidering their service strategies. Relevant data for the purpose of this study were collected through questionnaires from 383 respondents by using online channels. The online channels were exclusively employed for maintaining the safety of respondents during COVID-19. Respondents for this study were online shoppers who encountered service failure during COVID-19. The results indicated that the incidence of service failure has increased due to an increase in online shopping during COVID-19. Some customers tend to repurchase from the same webstore. On the other hand, some customers do not want to purchase again from the same seller and decided to switch to the alternative webstore. Based on the findings, new strategy for online shopping service providers was introduced. This strategy will be helpful for the online service providers to increase their profitability by retaining their dissatisfied customers. Service providers can minimize the number of customers switching to other webstores by reducing the events of service failure. Customer’s assistive intent can also be helpful for service providers to increase the efficiency of service recovery. Conducting a proper follow-up after providing service recovery can also reduce the switching of customer. It will be helpful for service providers to understand the customers’ expectations before recovery process and their feeling after getting service recovery.

A quadruple blinded placebo controlled randomised trial to evaluate the effectiveness of an Iodine complex for patients with mild to moderate COVID-19 in Pakistan (I-COVID-PK): A structured summary of a study protocol for a randomised controlled trial.

OBJECTIVES: The objective of the study is to measure the efficacy of ionic-iodine polymer complex [1] for clinical and radiological improvement in coronavirus disease 2019 (COVID-19) patients. TRIAL DESIGN: The trial will be closed label, randomized and placebo-controlled with a 1:1:1:1 allocation ratio and superiority framework. PARTICIPANTS: All PCR confirmed COVID-19 adult patients including non-pregnant females, with mild to moderate disease, will be enrolled from Shaikh Zayed Post-Graduate Medical Complex, Ali Clinic and Doctors Lounge in Lahore (Pakistan). Patients with any pre-existing chronic illness will be excluded from the study. INTERVENTION AND COMPARATOR: In this multi-armed study ionic-iodine polymer complex with 200 mg of elemental iodine will be given using three formulations to evaluate efficacy. Patients will be receiving either encapsulated iodine complex of 200 mg (arm A), iodine complex syrup form 40 ml (arm B), iodine complex throat spray of 2 puffs (arm C) or empty capsule (arm D) as placebo; all three times a day. All the 4 arms will be receiving standard care as per version 3.0 of the clinical management guidelines for COVID-19 established by the Ministry of National Health Services of Pakistan. MAIN OUTCOMES: Primary outcomes will be viral clearance with radiological and clinical improvement. SARS-CoV-2 RT-PCR and HRCT chest scans will be done on the admission day and then after every fourth day for 12 days or till the symptoms are resolved. RT-PCR will only be shown as positive or negative while HRCT chest scoring will be done depending on the area and severity of lung involvement [2]. Time taken for the alleviation of symptoms will be calculated by the number of days the patient remained symptomatic. 30-day mortality will be considered as a secondary outcome. RANDOMISATION: Stratification for initial COVID-19 status (or days from initial symptoms as a proxy), age groups, gender, baseline severity of symptoms and co-morbidities will be used to ensure that the study arms remain balanced in size for the 1:1:1:1 allocation ratio. Randomization will be done using the lottery method. As patients are being admitted at different times, they will be recruited after obtaining their voluntary written informed consent following all standard protocols of the infection, control and disinfection. BLINDING (MASKING): This is a quadruple (participants, care providers, investigators and outcomes assessors) blinded study where only the study's Primary Investigator will have information about the arms and their interventions. NUMBERS TO BE RANDOMISED (SAMPLE SIZE): 200 patients will be randomized into four groups with three experimental and one placebo arm. TRIAL STATUS: Protocol Version Number is 2.3 and it is approved from IRB Shaikh Zayed Hospital with ID SZMC/IRB/Internal0056/2020 on July 14th, 2020. The recruitment is in progress. It was started on July 30, 2020, and the estimated end date for the trial is August 15, 2021. TRIAL REGISTRATION: Clinical Trial has been retrospectively registered on www.clinicaltrials.gov with registration ID NCT04473261 dated July 16, 2020. FULL PROTOCOL: The full protocol is attached as an additional file, accessible from the Trials website (Additional file 1). With the intention of expediting dissemination of this trial, the conventional formatting has been eliminated; this Letter serves as a summary of the key elements of the full protocol. The study protocol has been reported in accordance with the Standard Protocol Items: Recommendations for Clinical Interventional Trials (SPIRIT) guidelines.

Clinical efficacy of iodine complex in SARS-CoV-2-infected patients with mild to moderate symptoms: study protocol for a randomized controlled trial.

BACKGROUND: Coronavirus disease 2019 (COVID-19) caused by the novel coronavirus-infected millions globally. Despite a wide range of advised options for the treatment of COVID-19, a single strategy to tackle this pandemic remains elusive, thus far. That is why we are conducting a clinical trial to find out the efficacy of iodine complex to clear a viral load of severe respiratory syndrome coronavirus-2 (SARS-CoV-2) along with a reduction in time taken to alleviate symptoms. METHOD: The proposed study is a placebo-controlled, add-on, randomized trial using parallel group designs. This is a closed-label and adaptive with sample size reassessment, multi-centered design with a 1:1:1:1 allocation ratio and superiority framework. It will be conducted in Shaikh Zayed Post-Graduate Medical Complex, Ali Clinic, and Doctors Lounge, Lahore, Pakistan. This study will have three arms of mild to moderately symptomatic COVID-19 patients (50 patients in each) which will receive ionic-iodine polymer complex with 200 mg of elemental iodine: interventional arm A will have encapsulated, arm B will receive suspension syrup form, arm C will get throat spray, while arm X will be standard care with placebo. Data will be collected on self-constructed, close-ended questionnaires after obtaining written consent. Data will be analyzed using SAS version 9.4. COVID-19 patients will be monitored by RT-PCR and HRCT (high-resolution computed tomography) chest. In addition to these, the duration of the symptomatic phase and mortality benefits will be analyzed in both groups. DISCUSSION: The study is designed to measure the superior efficacy of the iodine complex as an add-on in treating COVID-19-positive patients with mild to moderate symptoms. This combination is hypothesized to improve various parameters like rapid viral load reduction, clinical and radiological improvement, lower mortality, and reduction in hospitalization. The trial will aid in devising a better strategy to cope with COVID-19 in a relatively inexpensive and accessible way. The implications are global, and this could prove itself to be the most manageable intervention against COVID-19 especially for patients from limited-resource countries with deprived socioeconomic status. TRIAL REGISTRATION: ClinicalTrials.gov NCT04473261 . Registered on July 16, 2020.

Disparities in Health Care Delivery Systems.

Racial disparities in health care systems exist in all phases of health care delivery. The Affordable Care Act has been unable to completely mitigate disparities in health care as the root cause (ie, socioeconomic inequality) remains unaddressed. Uninsured status, lack of transportation, high costs, health literacy, provider unavailability, lack of trust in the health system, and implicit bias block minority populations from obtaining deserved quality care. With the COVID-19 crisis, increased sensitivity and development of innovative approaches to provide accessibly and quality health care are necessary.

Social distancing in the seminar room of a children’s hospital – a prospective QI analysis of practices and recommendations

BackgroundSARS-CoV-2 can be spread when people have close sustained contact. This means spending more than 15 minutes within two metres of a covid-positive person as it spreads through droplets from sneezes and cough. To that effect, Public Health England recommends social distancing, that is, maintaining a distance of at least 2 metres between people of different households. In cases where a 2m distance is not possible, additional measures such a wearing a face covering and having adequate ventilation should be ensured.ObjectivesThe objective of this audit was to look at social distancing measures among healthcare professionals during the morning paediatric handover in the seminar room at our Hospital and to identify areas of improvement so as to prevent the spread of the infection among hospital.MethodsA daily head count was carried out at the morning handover in the seminar room from 1st November 2020 to 15th January 2021. This included doctors, medical students and physician associates. Given the size of the seminar room, the infection control team recommends that the total number of people in the room should not exceed 15 at any given time.ResultsThe recommended total number of people was exceeded on 25 discrete days (54.3%) in that period time. Moreover, chairs spaced 2m apart to ensure safe distancing were moved closer by the healthcare professionals on a daily basis. This increases the risk of spread amongst the staff. After communicating the results with all the stakeholders and staff at UHL Children’s hospital, some positive changes were noticed.Display of the poster within the seminar room and on the entrance door to the seminar room, marking the distance on the floor, communicating the results with staff and regular reminder about the need of adherence to PHE guidance helped the practice of social distancing. Since implementing this change, total number of people reduced to 9 on an average with maximum of 11 over 2 weeks period.ConclusionsSocial distancing measures should be better adhered to reduce the risk of person to person transmission in a hospital setting. Participants were advised to limit the number of staff to one to two each from every ward to help minimise the number of attendees in the seminar room. Staff are being encouraged to leave after handing over jobs from their specific wards. Strict adherence to guidance with regular reminders during handovers about its implementation and reviewing PDSA cycles are key to sustain this improvement.

Incessant threat of COVID-19 variants: Highlighting need for a mix of FDA-approved artificial intelligence tools and community pharmacy services.

Seamless integration of artificial intelligence (AI) technology, especially FDA-approved AI tools, in community pharmacies can build increasingly effective and easy care pathways, thereby minimizing burden on healthcare system, and ensuring compliance to preventive measure through limiting hospital visits. In this regard, the WHO needs to enlist and provide guidance on most promising AI tools that can be implemented in community pharmacy settings.

Safety and Efficacy of Renin-Angiotensin-Aldosterone System Inhibitors in COVID-19 Population.

INTRODUCTION: The safety of renin-angiotensin-aldosterone system inhibitors (RAASi) among COVID-19 patients has been controversial since the onset of the pandemic. METHODS: Digital databases were queried to study the safety of RAASi in COVID-19. The primary outcome of interest was mortality. The secondary outcome was seropositivity improvement/viral clearance, clinical manifestation progression, and progression to intensive care units. A random-effect model was used to compute an unadjusted odds ratio (OR). RESULTS: A total of 49 observational studies were included in the analysis consisting of 83,269 COVID-19 patients (RAASi n = 34,691; non-RAASi n = 48,578). The mean age of the sample was 64, and 56% were males. We found that RAASi was associated with similar mortality outcomes as compared to non-RAASi groups (OR 1.07; 95% CI 0.99-1.15; p > 0.05). RAASi was associated with seropositivity improvement including negative RT-PCR or antibodies, (OR 0.96; 95% CI 0.93-0.99; p < 0.05). There was no association between RAASi versus control with progression to ICU admission (OR 0.99; 95% CI 0.79-1.23; p > 0.05) or higher odds of worsening of clinical manifestations (OR 1.04; 95% CI 0.97-1.11; p > 0.05). Metaregression analysis did not change our outcomes for effect modifiers including age, sex, comorbidities, RAASi type, or study type on outcomes. CONCLUSIONS: COVID-19 is not a contraindication to hold or discontinue RAASi as they are not associated with higher mortality or worsening symptoms. Continuation of RAASi might be associated with favorable outcomes in COVID-19, including seropositivity/viral clearance.

An in vitro antiviral activity of iodine complexes against SARS-CoV-2.

Since the emergence of COVID-19 pandemic in China in late 2019, scientists are striving hard to explore non-toxic, viable anti-SARS-CoV-2 compounds or medicines. We determined In vitro anti-SARS-CoV-2 activity of oral formulations (syrup and capsule)of an Iodine-complex (Renessans). First, cell cytotoxicity of Renessans on the Vero cells was determined using MTT assay. Afterwards, the antiviral activity of Renessans was determined using viral inhibition assays and TCID50. For this, nontoxic concentrations of the Renessans were used. The results showed that Renessans is nontoxic to the cells up to 50 µg/mL. At 1.5 µg/mL concentration, SARS-CoV-2 production was significantly reduced to 101.43 TCID50 and 101.58 TCID50 for the syrup and capsule, respectively, as compare to virus infected control cells 106.08 TCID50 and we found the dose dependent inhibition of virus replication in the presence of Renessans. Renessans inhibited SARS-CoV-2 with an EC50 value of 0.425 µg/mL and 0.505 µg/mL for syrup and capsule, respectively. Furthermore, there was no virus detected at concentration of 50 µg/mL of Renessans. This study indicates that Renessans, containing iodine, have potential activity against SARS-CoV-2 which needs to be further investigated in human clinical trials.

Vitamin D attenuates COVID-19 complications via modulation of proinflammatory cytokines, antiviral proteins, and autophagy.

INTRODUCTION: Global emergence of coronavirus disease-19 (COVID-19) has clearly shown variable severity, mortality, and frequency between and within populations worldwide. These striking differences have made many biological variables attractive for future investigations. One of these variables, vitamin D, has been implicated in COVID-19 with rapidly growing scientific evidence. AREAS COVERED: The review intended to systematically explore the sources, and immunomodulatory role of vitamin D in COVID-19. Search engines and data sources including Google Scholar, PubMed, NCBI, Scopus, and Web of Science were used for data collection. The search terms used were Vitamin D, COVID-19, immune system, and antiviral mechanism. Overall, 232 sources of information were collected and 188 were included in this review. EXPERT OPINION: Interaction of vitamin D and vitamin D receptor (VDR) triggers the cellular events to modulate the immune system by regulation of many genes. Vitamin D operates as a double-edged sword against COVID-19. First, in macrophages, it promotes the production of antimicrobial and antiviral proteins like ß-defensin 2 and cathelicidin, and these proteins inhibit the replication of viral particles and promote the clearance of virus from the cells by autophagy. Second, it suppresses cytokine storm and inflammatory processes in COVID-19.

COVID-19: Prospective Challenges and Potential Vaccines

ContextRNA viruses exhibit an extraordinary ability to evolve in a changing environment and to switch from animal hosts to humans. The ongoing COVID-19 pandemic, recognized as a respiratory disease, is an example of zoonotic transmission of the RNA virus known as SARS-CoV-2. The development and regulatory approval of a vaccine against SARS-CoV-2 pose multiple preventive and therapeutic challenges, especially during an ongoing pandemic.ObjectiveThe review intended to examine the challenges and recent achievements in the development of vaccine candidates against COVID-19.DesignThe research team performed a literature review, searching relevant and up to date information from the literature. The sources of data included Google Scholar, PubMed, NCBI, and Yahoo. The search terms used were COVID-19 challenges, SARS-CoV-2 prospective challenges, RNA viruses adoptability, host switching by RNA viruses, COVID-19 vaccines.SettingThe study took place at the digital libraries of contributing institutions. The data was combined, selected for further analysis and manuscript preparation at King Abdulaziz University.ResultsRNA viruses with high rate of genome alterations and evolution have better chances to survive in the adverse environmental conditions by adopting the alternate host species. The recent epidemics such as SARS, MERS, and COVID-19 are examples of zoonotic transmission of RNA viruses from animal species to the humans. However, the mechanisms involved in the switching-on to new host species need further investigations to control the zoonotic transmissions in near future. As of April 2020, 115 candidate vaccines were being evaluated;78 of them had been found to be active, and a few of them are in Phase I trials. In the development of different types of vaccine candidates against COVID-19, multiple international pharmaceutical and biotechnology companies are involved.ConclusionsEmerging and re-emerging pathogenic RNA viruses pose a serious threat to human health. Little is known about the human-host adoptive mechanism for zoonotic transmission. Deep insights into the molecular mechanism responsible for the switching of animal or bird viruses to humans could provide target molecules or events to prevent such transmissions in the near future. Fast development and approval of efficacious and safe vaccines is key to the effort to provide preventive measures against COVID-19 and future viruses. However, the development and availability of a vaccine candidate is a time-consuming process and often can't be completed during an epidemic. Currently, several types of vaccines are under development, and most of them won't realistically be available in time for the present COVID-19 pandemic.

A study of community knowledge, attitudes, practices, and health in Pakistan during the COVID-19 pandemic.

The ongoing outbreak of novel Coronavirus originated from Wuhan, China, and vigorously spread throughout the globe causing serious concerns. This study aimed to appraise the community demographics, knowledge, attitudes, practices mental health, symptoms, and precautionary measures during the COVID-19 pandemic in Pakistan. This study was a cross-sectional and observational study. An online semi-structured questionnaire was designed by freely available Google Forms. A Referral Sampling technique was used, a total of 439 responses were recorded and analyzed by using Statistical Package for the Social Sciences. Regression analysis and multinomial logistic regression analysis were done to test the hypothesis. This study shows more than 63% of participants maintain social distancing at their workplaces, offices, markets, and so forth and 59% of participants stayed at their homes during the lockdown. Almost 75% of participants did not attend any gathering during COVID-19. Most participants (75.4%) wear masks and gloves, and 85.6% of participants wash their hands with soap and using hand sanitizer regularly. This study shows that 40.8% and 36.2% of participants felt anxiety and fear due to Coronavirus. This study shows that knowledge among the population in Pakistan about COVID-19 was low. Continued efforts are needed to address knowledge, attitudes, and practices to interrupt novel Coronavirus transmission.